Research the literature on your own
- Learn about the basic principles underlying mass spectrometry.
- Several Clb5-specific substrates (Orc2, Orc6, Cdc6, Mcm3) form a large protein complex (called the pre-replicative complex or pre-RC) at origins of replication. Why is it important for these proteins to be phosphorylated by Clb5-Cdk complexes at the onset of S phase?
- One Clb5-specific substrate (Sld2) is a component of a large ‘pre-initiation’ complex of proteins that forms at origins of replication. Learn about how phosphorylation of this protein (and a related protein called Sld3) is important for the initiation of chromosome duplication.
- One of the Clb5-specific proteins is called Fin1. What is the function of Fin1, and why is Fin1 a better substrate for Clb5 than it is for Clb2?
- Phosphatases reverse the effects of Cdks in the cell. In the budding yeast, the phosphatase Cdc14 is the key regulator of Cdk substrates in late mitosis. Learn how Cdc14 is regulated in late mitosis, and how it is thought to promote various late mitotic events.
- The proteins Sic1 and Cdh1 are excellent examples of proteins that are regulated by phosphorylation by Cdk1 at multiple sites. Learn how the multi-site phosphorylation of these proteins regulates their function.
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