Review Questions

1. What is a control experiment?
   
   
2. What is GFP? What is its value? How does it work?
   
   
3. What is a reporter? How does GFP differ from Luciferase?
4. What are the advantages/disadvantages of live versus fixed embryo imaging?
   
   
5. How would you convince yourself and others that attaching GFP (~25kd) to Bicoid (~55kd) does not change its normal behaviour (in other words, that the fusion is functional)?
   
   
6. Scientific discovery is often driven by advances in technology and methodology. Can you identify tools, techniques and/or methods that were used by Eric Wieschaus and his colleagues that were not available 20 years ago? Can you explain how they enabled EW to address the questions he is asking?
   
   
7. If the embryo is homozygous mutant for the gene bicoid it develops normally. Can you explain that?
   
   

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